Fig. 1: EVA1A expression profile and autophagy in mice with D–GalN/LPS-induced acute liver failure.

Mice were intraperitoneally injected with d-GalN (350 mg/kg) and LPS (30 μg/kg) and killed at 2, 4, or 6 h (n = 5). The mice in the control group (n = 4) were injected with PBS only. a The relative levels of Eva1a mRNA were measured by qRT-PCR in the livers (*P < 0.05, ***P < 0.001, n = 4). b The levels of Eva1a protein were measured by Western blot in the livers. A representative blot from two samples of every group is shown. c The quantification of Eva1a levels relative to Gapdh treated as described in b. The average value of PBS-treated mice was normalized to 1 (*P < 0.05, n = 4). d Eva1a expression was detected by an immunohistochemical analysis of the liver tissues. Scale bar = 100 µm. e Isotype IgG staining of liver tissues. f The levels of Lc3b, Atg12-5, Atg16l1, Becn-1, and Eva1a were measured by a Western blot assay of the livers. A representative blot from two samples of every group is shown. g Quantification of the indicated protein levels relative to Gapdh treated as described in (f). The average value for the PBS-treated mice was normalized to 1 (*P < 0.05, **P < 0.01, n = 4)