Fig. 2: Generation of liver-specific Eva1a−/− mice. | Cell Death & Disease

Fig. 2: Generation of liver-specific Eva1a−/− mice.

From: Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy

Fig. 2

a Scheme used to generate Eva1a deficient mice. b Genomic DNA was extracted from mouse tails and analyzed by PCR. c Representative RT-PCR results showing endogenous Eva1a expression in different tissues from Eva1af/f and Eva1a-deficient mice. d The levels of Eva1a protein were detected by Western blot assays. e Eva1a expression was detected by immunohistochemical analysis. Isotype IgG staining was used as a negative control. Scale bar = 50 µm

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