Fig. 4: Deletion of Eva1a results in mitochondrial damage and increased apoptosis in mice with ALF.
From: Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy
a Ultrastructural images reveal the accumulation of swollen mitochondria in d-GalN/LPS treated Eva1a−/− mice. Scale bar = 5 µm or 2 µm. b Detection of ATP levels in the livers from different groups (*P < 0.05, n = 3). c Representative images of TUNEL staining (green) and Hoechst staining (blue) of the nuclei in liver tissues. Scale bar = 100 µm. d Quantification of TUNEL-positive cells per 1000 nuclei (***P < 0.001). e Representative Western blot of Pro-caspase 3, Cleaved caspase 3, Pro-caspase 6 and Parp in the liver extracts obtained from different groups of mice. f Quantification of indicated protein levels relative to Gapdh treated as described in e. Average value of Eva1a+/+ mice without d-GalN/LPS was normalized to 1 (*P < 0.05, n ≥ 3)
