Fig. 2: Infusion of hUC-MSCs promoted restoration of pancreatic islets function in T2D mice. | Cell Death & Disease

Fig. 2: Infusion of hUC-MSCs promoted restoration of pancreatic islets function in T2D mice.

From: Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice

Fig. 2

a Apoptosis of insulin producing cells (red) was evaluated by TUNEL labeling (green), and number of TUNEL+ β-cells/islet was calculated. The arrow: insulin+ cells showing TUNEL expression. b Representative islets stained with antibodies against insulin (green) and Ki67 (red) of the Con, T2D and the hUC-MSCs group. Number of Ki67+ β-cells/islet was calculated. The arrow: insulin+ cells showing Ki67 expression. c Photomicrographs double stained with anti-insulin (green) and anti-Pdx-1 (red) antibodies of the three groups. The percentage of insulin producing cells co-expressing Pdx-1 was quantified. d Photomicrographs double stained with anti-insulin (green) and anti-MafA (red) antibodies of the three groups. The percentage of insulin producing cells co-expressing MafA was quantified. Scale bar, 25 μm. Quantification was determined by evaluating islets from at least 5 sections of each group. n = 5 mice per group. The results were presented as the means ± SD. **p < 0.01. TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling

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