Fig. 7: HUC-MSCs modulated macrophage polarization via secretion of IL-6 in vivo. | Cell Death & Disease

Fig. 7: HUC-MSCs modulated macrophage polarization via secretion of IL-6 in vivo.

From: Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice

Fig. 7

Photomicrographs of representative islets stained with anti-Fizz1 (red) (a) and anti-CD163 (red) (c) antibodies from the T2D mice without (T2D group) or with the administration of hUC-MSCs transfected with scrambled siRNA (MSCs/scr siRNA group) or hUC-MSCs transfected with IL-6 siRNA (MSCs/IL-6 siRNA group). The dotted line circled areas are pancreatic islets. Quantifications of Fizz1+ cells and CD163+ cells within islets were determined by evaluating islets from at least five sections of each group, results of which were separately presented in b and d. e Blood glucose level was determined consecutively after STZ injection. Values are means ± SD. **p < 0.01 for the MSCs/scr siRNA group versus the T2D group. #p < 0.05 for MSCs/IL-6 siRNA group versus the MSCs/scr siRNA group. f Glucose tolerance was assessed by IPGTT. ∫∫, blood glucose level exceeded the maximum (36 mmol/l) of the glucometer. g Photomicrographs double stained with anti-insulin (green) and anti-Pdx-1 (red) antibodies of the three groups. The percentage of insulin-producing cells co-expressing Pdx-1 was quantified by evaluating islets from at least five sections of each group, results of which were shown in h. Scale bar, 50 μm. n = 5 mice per group. The results were presented as the means ± SD. *p < 0.05, **p < 0.01

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