Fig. 8: HUC-MSCs secreted MCP-1 to coordinate with IL-6 in regulating macrophage polarization. | Cell Death & Disease

Fig. 8: HUC-MSCs secreted MCP-1 to coordinate with IL-6 in regulating macrophage polarization.

From: Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice

Fig. 8

a Quantitative RT-PCR analysis of MCP-1 expression in hUC-MSCs. HUC-MSCs were cultured with M0 or M1 for 6, 12, 24 h. Results were presented relative to those of UC- MSCs cultured with M0, set as 1. b Secretory level of MCP-1 in medium of hUC-MSCs (Con), hUC-MSCs conditioned with IgG (MSCs/IgG) or hUC-MSCs conditioned with MCP-1 neutralizing antibody (NA) (MSCs/MCP-1 NA). HUC-MSCs were cultured with M0 or with M1. c Immunofluorescence of iNOS+-BMDMs and Fizz1+-PMs in stimulation with LPS and IFN-γ cultured alone, with hUC-MSCs conditioned with IgG, hUC-MSCs conditioned with MCP-1 neutralizing antibody (NA), or hUC-MSCs conditioned with MCP-1 neutralizing antibody (NA) plus IL-6 NA. Scale bar, 100 μm. Quantification of iNOS+ BMDMs and Fizz1+ PMs were presented in d and e. f Immunoblotting analysis of iNOS and Arg1 in BMDMs of the same four groups mentioned in c. Relative protein level is quantified by ratio of iNOS to β-tubulin g and that of Arg1 to β-tubulin h. Values are means ± SD of three individual experiments, *p < 0.05, **p < 0.01

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