Fig. 2: SLC15A3 was regulated by the activation of NF-κB and the promotor region of SLC15A3 contained potential NF-κB binding sites. | Cell Death & Disease

Fig. 2: SLC15A3 was regulated by the activation of NF-κB and the promotor region of SLC15A3 contained potential NF-κB binding sites.

From: Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage

Fig. 2

a, b mRNA and protein expression of Slc15a3 in mouse PMs (a) and BMDMs (b) pretreated with or without 10 μM BAY 11–7082 (BAY) for 1 h, and then treated with or without 100 ng/mL LPS for another 4 h or 24 h for mRNA and protein detection, respectively. Quantification of protein (i.e., SLC15A3/GAPDH ratio) is shown in the right side of each western blot figure. One-way ANOVA followed by Tukey’s test was used to evaluate the statistical differences, *P < 0.05, **P < 0.01 and ***P < 0.001. c Mouse Slc15a3 promotor constructs of varying length were fused to the luciferase reporter gene in the pGL3-basic vector. Predicted binding sites are shown schematically (black boxes), where the binding site numbers indicate position 5′ upstream of the start codon. d Luciferase activity in HeLa cells transfected with luciferase reporter constructs (horizontal line), with or without pcDNA3.1-mNF-κB, were normalized to renilla luciferase activity and presented relative to those transfected with pcDNA3.1 cells. Statistical analyses were performed using an unpaired t test. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the vehicle group. The data are expressed as mean ± SE (n = 3)

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