Fig. 3: Blocking the MAPK signaling pathway diminished LPS-induced SLC15A3 upregulation. | Cell Death & Disease

Fig. 3: Blocking the MAPK signaling pathway diminished LPS-induced SLC15A3 upregulation.

From: Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage

Fig. 3

Mouse PMs (left panel) and BMDMs (right panel) were pretreated with or without 50 μM U0126, 10 μM SB203085 (SB203) and 10 μM SP600125 (SP600) for 1 h, then the cells were treated with or without 100 ng/mL LPS for another 4 h. mRNA expression of Slc15a3 (a, b), Il-6 (c, d) and Tnf-α (e, f) were determined by real-time PCR. One-way ANOVA followed by Tukey’s test was used to evaluate the statistical differences, *P < 0.05, **P < 0.01, and ***P < 0.001. The data are expressed as mean ± SE (n = 3)

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