Fig. 5: SLC15A3 expression was upregulated by TLR2 and TLR7 ligands. | Cell Death & Disease

Fig. 5: SLC15A3 expression was upregulated by TLR2 and TLR7 ligands.

From: Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage

Fig. 5

a–d mRNA and protein expression of Slc15a3 in mouse PMs (a, b) and BMDMs (c, d) treated with 1 μg/mL LTA or 5 μg/mL R837 for specified periods of time. Quantification of protein (i.e., SLC15A3/GAPDH ratio) is shown below of each Western blot figure. One-way ANOVA followed by Dunnett’s test was used to evaluate the statistical differences. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with 0 h. e, f Mouse PMs (e) and BMDMs (f) were pretreated with or without 10 μM BAY 11–7082 (BAY) for 1 h, then treated with or without 1 μg/mL LTA or 5 μg/mL R837 for 4 h. mRNA expression of Slc15a3 were determined by real-time PCR. One-way ANOVA followed by Tukey’s test was used to evaluate the statistical differences, *P < 0.05, **P < 0.01 and ***P < 0.001. The data are expressed as mean ± SE (n = 3)

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