Fig. 6: Knockdown or overexpression of SLC15A3 influenced the TLR4-dependent proinflammatory cytokine production. | Cell Death & Disease

Fig. 6: Knockdown or overexpression of SLC15A3 influenced the TLR4-dependent proinflammatory cytokine production.

From: Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage

Fig. 6

a mRNA and protein expression of SLC15A3 was determined in THP-1 cells transfected with 30 nM control siRNA (Ctrl) or two different SLC15A3 siRNA (#1 and #2) after 24 h transfection. Quantification of protein (i.e., SLC15A3/GAPDH ratio) is shown in the right side of each Western blot figure. b THP-1 cells transfected with 30 nM control siRNA (Ctrl) or SLC15A3 siRNA (#1). Twenty-four hours after transfection, the cells were treated with 100 ng/mL LPS for 12 h. The levels of IL-6 and TNF-α in the cell culture supernatants were determined by ELISA assays. c, d A549 cells transfected with 1 μg pcDNA3.1 or human SLC15A3 plasmid. Twenty-four hours after transfection, the cells were treated with 1 μg/mL LPS for 8 h. The mRNA (c) and protein (d) levels of IL-6 in cells and cell culture supernatants were determined by real-time PCR and ELISA assays. Statistical analyses were performed using an unpaired t test. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with control (Ctrl). The data are expressed as mean ± SE (n = 3)

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