Fig. 8: SLC15A3 expression was increased in mice with E. coli-induced peritonitis, and positively related to inflammation. | Cell Death & Disease

Fig. 8: SLC15A3 expression was increased in mice with E. coli-induced peritonitis, and positively related to inflammation.

From: Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage

Fig. 8

Mice were intraperitoneally injected with PBS or E.coli (1 × 107 cfu/mL, 0.5 mL/ 25 g), and the serum or peritoneal macrophages collected after 24 h. a IL-6 and TNF-α protein levels in mouse serum were determined by ELISA assays. b, c Slc15a3 mRNA (b) and protein expression (c) in PMs was determined by real-time PCR and Western blotting. Quantification of protein (i.e., SLC15A3/GAPDH ratio) is shown in the right side of each western blot figure. d mRNA expression of Il-6 and Tnf-α in peritoneal macrophages as a function of time. e Correlation analysis of Slc15a3 with Il-6 or Tnf-α was performed by linear regression, in which Slc15a3 mRNA expression in x-axis and Il-6 (left) or Tnf-α (right) mRNA expression in y-axis. Statistical analyses were performed using an unpaired t test. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with PBS group. The data are expressed as mean ± SE (n = 4–6)

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