Fig. 2: VJ suppresses cell growth in AKT-overexpressing HCT 116 cells. | Cell Death & Disease

Fig. 2: VJ suppresses cell growth in AKT-overexpressing HCT 116 cells.

From: Suppression of Notch1 and AKT mediated epithelial to mesenchymal transition by Verrucarin J in metastatic colon cancer

Fig. 2

a Western blot analysis of basal expression of AKT and pAKT (Ser473) in HCT 116 (pCMV and AKT) cells. b BrdU incorporation assay demonstrating cell proliferation of HCT 116 stable transfectants (AKT-6 and AKT-12). c Colony forming assay on HCT 116 stable transfectants. d HCT 116 stable transfectants were treated with indicated concentration of VJ or Vehicle (DMSO) for 24 h followed by MTT assay for cell viability. Student’s t-test was used to calculate statistical significance between vehicle and treatment at each concentration; *p < 0.05, **p < 0.01 and ***p < 0.001

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