Fig. 3: Inhibition of AKT signaling and induction of apoptosis by VJ in HCT transfectants. | Cell Death & Disease

Fig. 3: Inhibition of AKT signaling and induction of apoptosis by VJ in HCT transfectants.

From: Suppression of Notch1 and AKT mediated epithelial to mesenchymal transition by Verrucarin J in metastatic colon cancer

Fig. 3

a Total protein lysates from VJ-treated pCMV/HCT 116 and AKT/HCT 116 cells were analyzed for prosurvival markers pAKT, NFκB (p65), and Bcl-2. b Total protein lysates from VJ-treated pCMV/HCT 116 and AKT/HCT 116 cells were analyzed for pro-apoptotic marker cleaved PARP expression. c Apoptotic assays were performed using Annexin V–FITC and PI staining in non-transfected and AKT-overexpressing HCT 116 transfectants treated with the indicated concentration of VJ or vehicle (DMSO). Student’s t-test was used to calculate statistical significance between vehicle and treatment at each concentration; *p < 0.05 and ***p < 0.001

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