Fig. 4: VJ down-regulates Notch1 in HCT 116 transfectants. | Cell Death & Disease

Fig. 4: VJ down-regulates Notch1 in HCT 116 transfectants.

From: Suppression of Notch1 and AKT mediated epithelial to mesenchymal transition by Verrucarin J in metastatic colon cancer

Fig. 4

a Western blot analysis of VJ-treated HCT 116 and SW-620 cells for Notch1 and Presenilin 1 expression. b, c Immunoblot analysis for the expression of Notch1 and Presenilin 1 in AKT/HCT 116 stable clones and Notch/HCT 116 cells. d Stable clones from HCT 116 (pCMV and Notch1) were treated with the indicated concentration of VJ or vehicle (DMSO) for 24 h followed by the MTT assay. Student’s t-test was used to calculate statistical significance between vehicle and treatment at each concentration; ***p < 0.001

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