Fig. 2: Loss of DDHD2 increases apoptosis sensitivity of MEFs. | Cell Death & Disease

Fig. 2: Loss of DDHD2 increases apoptosis sensitivity of MEFs.

From: Loss of DDHD2, whose mutation causes spastic paraplegia, promotes reactive oxygen species generation and apoptosis

Fig. 2

a Lysates (10 µg) of WT and DDHD2 KO MEFs were analyzed by WB with antibodies against DDHD2 and GAPDH. b, c WT and DDHD2 KO MEFs were treated with 1 µM STS for 8 h, followed by staining with TUNEL and Hoechst 33342 (b), or antibodies against Bax and cytochrome c (c). The number of TUNEL-positive cells or cells exhibiting dot-like Bax staining or cytosolic cytochrome c staining was determined. d DDHD2 KO MEFs were transfected with a plasmid encoding FLAG (Empty), FLAG-DDHD2 WT, or FLAG-DDHD2-S351A. At 24 h after transfection, STS was added to a final concentration of 1 µM, and the cells were incubated for 8 h, and then subjected to staining with TUNEL and an anti-FLAG antibody. The number of TUNEL-positive cells was determined. No significant difference in the percentage of TUNEL-positive cells was observed between cells transfected with FLAG and FLAG-DDHD2-S351A. Data represent means ± SEM for nine (b) and three (c, d) independent experiments, respectively. The scale bars for whole cell panels are 10 μm. *P < 0.05, **P < 0.01, Student’s t-test

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