Fig. 6: Reduction of CLs in DDHD2 KO MEFs.

a The CL species in WT and DDHD2 KO MEFs were analyzed by mass spectrometry, as described under Materials and methods. The bar graph shows the total amounts of CLs. b WT and DDHD2 KO MEFs were incubated with 1 µM NAO and 500 nM MitoTracker Red FM for 30 min, and then analyzed by IF microscopy. The scale bar is 10 μm. The fluorescence intensity of NAO was quantified and is expressed as the ratio relative to that of WT MEFs. Data in this figure represent means ± SEM (N = 3). **P < 0.01, Student’s t-test