Fig. 7: DDHD2 partially localizes to mitochondria and becomes aggregated in response to oxidative stress.

a WT MEFs were untreated (Un) or treated with 2 µM antimycin A and 1 µM rotenone (AR), 0.1 mM PQ, 0.1 mM t-BHP or 1 µg/ml tunicamycin (Tm) for 2 h, followed by immunostaining with antibodies against DDHD2 and cytochrome c. The number of cells with DDHD2 dots was determined. Data represent means ± SEM (N = 3). *P < 0.05, **P < 0.01, Student’s t-test. The scale bars are 10 μm. b, c DDHD2 KO MEFs with stable expression of DDHD2-WT-mCherry or DDHD2-S351A-mCherry were treated with 0.1 mM t-BHP for 30 min, and then incubated with 0.1 µM MitoPeDPP for 15 min and analyzed by time-lapse imaging. The scale bar is 1 μm. See Supplementary Movies 1 and 2