Fig. 5: α-Syn-induced increase in LAMP-2A is not changed by ATG5 siRNA.
From: Exosomal secretion of α-synuclein as protective mechanism after upstream blockage of macroautophagy
The effect of α-Syn overexpression and ATG5 siRNA on markers for chaperone-mediated autophagy was analyzed in naïve control cells (Ctrl), cells overexpressing α-Syn, cells overexpressing the control protein GFP, cells overexpressing α-Syn and treated with ATG5 siRNA, and naïve cells treated with ATG5 siRNA. β-actin was used as loading control. a Representative Western blot for LAMP-2A. b Quantification of LAMP-2A, normalized to β-actin, from Western blots as shown in a, showing an α-Syn-induced LAMP-2A increase, which was not changed by ATG5 siRNA. c Representative Western blot for HSC70. d Quantification of HSC70, normalized to β-actin, from Western blots as shown in c, showing stable expression across the experimental conditions. Data in b, d are mean ± standard error from n ≥ 3 biological replicates. n.s., not significant, ***p < 0.001; one-way analysis of variance with Bonferroni’s post hoc test
