Fig. 7: The nucleic accumulation of β-catenin was positively correlated with CD68⁺ TAMs in HCC patient biopsies.

a Human HCC biopsies were sectioned and stained with anti-CD68 (Cy5), anti-β-catenin (FITC), and anti-Arg1 (Cy3) using immunofluorescence staining, and counterstained with Hoechst. The representative images of CD68⁺β-catenin⁺Arg1⁺ cells in HCC sections were observed (upper panel) and shown with high magnitude (lower panel) (n = 25). b The expression of β-catenin and Arg1 in randomly selected CD68⁺ cells in (a) were quantified using Mean Density (IOD/area). The correlation of the nucleic β-catenin expression with Arg-1⁺CD68⁺ TAMs in HCC patients was analyzed. c Human HCC biopsies were sectioned and stained with anti-CD68 (Cy5), anti-β-catenin (FITC), and anti-MR (Cy3) using immunofluorescence staining, and counterstained with Hoechst. The representative images of CD68⁺β-catenin⁺MR⁺ cells in HCC sections were observed (upper panel) and shown with high magnitude (lower panel) (n = 25). d The expression of β-catenin and MR in randomly selected CD68⁺ cells in (c) were quantified using Mean Density (IOD/area). The correlation of the nucleic β-catenin expression with MR⁺CD68⁺ TAMs in HCC patients was analyzed