Fig. 6: BAP31 affect cells migration and invasiveness by regulating the expressions and subcellular localization of the metastasis-related proteins Drebrin, M-RIP, SPECC1L, and Nexilin. | Cell Death & Disease

Fig. 6: BAP31 affect cells migration and invasiveness by regulating the expressions and subcellular localization of the metastasis-related proteins Drebrin, M-RIP, SPECC1L, and Nexilin.

From: BAP31, a newly defined cancer/testis antigen, regulates proliferation, migration, and invasion to promote cervical cancer progression

Fig. 6

a Co-immunoprecipitation experiments showing the interaction between BAP31 and metastasis-related proteins, such as Drebrin, M-RIP, SPECC1L, and Nexilin. HeLa cells extracts were immunoprecipitated with FM-1 and an irrelevant control antibody. The immunoprecipitates were analyzed by western blotting using anti-BAP31, anti-Drebrin, anti-M-RIP, anti-SPECC 1, and anti-Nexilin antibodies. b Western blotting results to detect the expression levels of Drebrin, M-RIP, SPECC 1, and Nexilin in control and BAP31-depleted groups of HeLa cells. c Immunofluorescence staining of HeLa cells with anti-BAP31, anti-BAP31, anti-Drebrin, anti-M-RIP, anti-SPECC 1, and anti-Nexilin antibodies in control and BAP31-depleted groups. The scale bars represent 5 μm. d Western blotting results showing the expression levels of BAP31 in HeLa cells transfected with Drebrin, M-RIP, SPECC1L, or Nexilin siRNA. e Wound-healing assays were performed to evaluate the migration of HeLa cells transfected with BAP31, Drebrin, M-RIP, SPECC1L, and Nexilin or control siRNA. The scale bars represent 100 μm. f Transwell invasion assays of HeLa and SiHa cells treated as in (e). The results represent means ± SD from five microscopic fields. The scale bars represent 100 μm. **p < 0.01 was considered significantly different for each group vs siCon group

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