Fig. 4: ZDHHC8 may alter the postsynaptic glutamatergic receptor response probability.

a, b Representative traces showing mEPSCs (a) and mIPSCs (b) recorded from CA1 pyramidal cells. c, d Cumulative probability plots of mEPSC (c) and mIPSC amplitudes (d). Insets show bar graphs of the amplitudes (mEPSCs: rAAV-ZDHHC8-sh, 18 cells; rAAV-Scr-sh, 12 cells; Control, 12 cells; rAAV-Empty-GFP, 12 cells; and rAAV-ZDHHC8, 18 cells; n = 4 mice per group; left, ***P < 0.001, right, *P < 0.05); mIPSCs: rAAV-ZDHHC8-sh, 18 cells; rAAV-Scr-sh, 15 cells; Control, 20 cells; rAAV-Empty-GFP, 18 cells; and rAAV-ZDHHC8, 18 cells; n = 4 mice per group). e, f Cumulative probability plots of mEPSC (e) and mIPSC (f) frequencies (measured by inter-event intervals). Insets display bar graphs of frequencies (the numbers of cells in c, d). Data represent the mean ± SEM. Statistical significance was evaluated with the KS-test (cumulative probability plots) and one-way ANOVA (*P < 0.05, ***P < 0.001; N.S. represents no significance). g, h Localization of ZDHHC8 in the hippocampus of chronic epileptic mice (g) and in the neocortex of TLE patients (h). a–c ZDHHC8 was expressed (green) in the cell membrane but not the cytoplasm. d–g ZDHHC8 expression (green) and PSD95 (red) were co-localized, indicating that ZDHHC8 was located at excitatory synapses. h–k ZDHHC8 expression (green) and VGLUT1 (purple) were not co-localized, indicating that ZDHHC8 was located in postsynaptic membranes. l–o ZDHHC8 expression (green), GAD67 (blue), and Gephyrin (red) were not co-localized, indicating that ZDHHC8 was not located in inhibitory synapses; scale bar, 50 μm. The white arrows in all the images indicate positive neurons