Fig. 3: Knockdown of spinal Munc13-1 expression decreases SNL-enhanced mEPSCs frequency in the ipsilateral dorsal horn. | Cell Death & Disease

Fig. 3: Knockdown of spinal Munc13-1 expression decreases SNL-enhanced mEPSCs frequency in the ipsilateral dorsal horn.

From: Spinal TNF-α impedes Fbxo45-dependent Munc13-1 ubiquitination to mediate neuropathic allodynia in rats

Fig. 3

a Representative traces and statistical analysis of miniature EPSCs (mEPSCs) recorded in the ipsilateral dorsal horn of spinal slices that were dissected from animals receiving the sham operation (Sham 7D) or SNL (SNL 7D) and SNL slices treated with missense siRNA or Munc13-1 siRNA (SNL 7D + MS siRNA or SNL 7D + Munc13-1 siRNA, respectively; 3 μg, 10 μL; once daily at days 3–6 after SNL). The inter-event interval of mEPSCs was significantly shorter and the amplitude was larger in the SNL than in the sham-operated group. Administering Munc13-1 siRNA to SNL rats elongated the inter-event interval without affecting the amplitude of mEPSCs (Kolmogorov–Smirnov test). **P < 0.01 compared with Sham 7D. ##P < 0.01 compared with SNL 7D (n = 4–6). Results were expressed as mean ± SEM

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