Fig. 8: TNF-α increased both the frequency and amplitude of the mEPSCs recorded from dorsal horn neurons. | Cell Death & Disease

Fig. 8: TNF-α increased both the frequency and amplitude of the mEPSCs recorded from dorsal horn neurons.

From: Spinal TNF-α impedes Fbxo45-dependent Munc13-1 ubiquitination to mediate neuropathic allodynia in rats

Fig. 8

a Representative traces and statistical analysis of miniature EPSCs (mEPSCs) recorded in the ipsilateral dorsal horn of spinal slices showing that spinal administration of TNF-α to naive (naive) rats (TNF-α, 1 pM, 10 μL) increased both the frequency and amplitude of the mEPSCs. Pretreatment with TNF-α-neutralizing antibody (TNF-α Ab + TNF-α; 100 ng, 10 μL) reversed the TNF-α-increased frequency and amplitude, but Munc13-1 siRNA (Munc13-1 siRNA + TNF-α; 3 μg, 10 μL; once daily for 4 days) reversed the TNF-α-increased frequency without affecting the TNF-α-increased amplitude (Kolmogorov–Smirnov test). **P < 0.01 compared with naive. ##P < 0.01 compared with TNF-α (n = 4–6). Results were expressed as mean ± SEM

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