Fig. 2: MSCs ameliorate HG-induced mitophagy inhibition. | Cell Death & Disease

Fig. 2: MSCs ameliorate HG-induced mitophagy inhibition.

From: Mesenchymal stem cells ameliorate hyperglycemia-induced endothelial injury through modulation of mitophagy

Fig. 2

a, b Colocalization between LC3 and COXIV was used as measure of mitophagy in the different groups, PCC > 0.5 signify detection correlation. c HUVECs were treated with HG, HG/MSCs or HG/rapamycin for 72 h; rapamycin (Rapa, 15 nM) was administered as a positive control to induce autophagy; and CQ was added 8 h before determining the autophagic flux. d HUVECs were pretreated with 3-MA (5 mM) for 4 h and then co-cultured with MSCs or HSFs in HG for 72 h, and the expression of LC3-II and P62 were detected by Western blot. Data are shown as the means ± SD from three independent experiments. (*P < 0.05 vs. Con, #P < 0.05 vs. CQ, &P < 0.05 vs. HG, $P < 0.05 vs. MSCs/HG, ^P < 0.05 vs. Rapa/HG)

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