Fig. 3: MIR100HG induced cell cycle arrest at G1/S via p21/p27 regulation. | Cell Death & Disease

Fig. 3: MIR100HG induced cell cycle arrest at G1/S via p21/p27 regulation.

From: LncRNA MIR100HG promotes cell proliferation in triple-negative breast cancer through triplex formation with p27 loci

Fig. 3

a, b Gene ontology analysis (a) and KEGG pathway analysis (b) for RNA-seq data. c Heatmap of 21 coding genes related to the cell cycle with significantly altered expression following knockdown of MIR100HG in MDA-MB-231 cells. KD1-1 and KD1-2 are two biological replicates of sh-MIR100HG-1; KD2-1 and KD2-2 are two biological replicates of sh-MIR100HG-2. d, e Effect of pCDH-MIR100HG on expressions of p27, p21, and cyclin D1 at the protein (d) and RNA (e) levels. f, g Effect of sh-MIR100HG on expressions of p27, p21, and cyclin D1 at the protein (f) and RNA (g) levels. h Detection of p27 expression by western blot analysis using xenograft assay samples. The data represent three independent experiments (d–g). *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA

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