Fig. 5: COL10A1 facilitates EMT, lung metastasis, and intestinal and peritoneal metastasis in GC in vivo. | Cell Death & Disease

Fig. 5: COL10A1 facilitates EMT, lung metastasis, and intestinal and peritoneal metastasis in GC in vivo.

From: TGF-β1-SOX9 axis-inducible COL10A1 promotes invasion and metastasis in gastric cancer via epithelial-to-mesenchymal transition

Fig. 5

a Nude mice were injected with tumor cells through the tail vein (n = 10 in each group). The weights of the mice, lung weight and number of metastatic nodules in the lung were counted (right). b Representative figures of metastatic nodules in the lungs were observed under a microscope. c Paraffin-embedded lung metastasis nodules were stained with anti-SOX9, anti-COL10A1, anti-E-cadherin or anti-Vimentin antibodies using IHC. d Nude mice were injected with tumor cells through the abdominal cavity to evaluate the intestinal and peritoneal metastatic potential of the cells (n = 6 in each group). e The intestinal metastasis and peritoneal metastasis per group were counted. f Paraffin-embedded peritoneal metastasis sections were stained with anti- COL10A1, anti-Ki67, anti-E-cadherin, anti-Vimentin, anti-CD31 or anti-CD34 antibodies using IHC. Scale bars represent 100 μm in (a), 60 μm in (c) and 20 μm in (f)

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