Fig. 2: Effect of BBR treatment on cholesterol-induced blockade of autophagic flux in HepG2 cells. | Cell Death & Disease

Fig. 2: Effect of BBR treatment on cholesterol-induced blockade of autophagic flux in HepG2 cells.

From: Berberine ameliorates blockade of autophagic flux in the liver by regulating cholesterol metabolism and inhibiting COX2-prostaglandin synthesis

Fig. 2

a Confocal microscopy examination of HepG2 cells expressing a tandem GFP-RFP-LC3 fusion protein treated with cholesterol (Chol, 50 µg/ml), chloroquine (CQ, 50 µg/ml), berberine (BBR, 20 µg/ml), or their combinations for 24 h. b LC3B-II and p62 protein levels in HepG2 cells treated with CQ, cholesterol (Chol), or a combination of CQ and cholesterol for 24 h. c LC3B-II and p62 protein levels in HepG2 cells treated with cholesterol (Chol), berberine (BBR), or a combination of cholesterol and BBR for 24 h. d LC3B-II and p62 protein levels in HepG2 cells treated with CQ, BBR, or their combination for 24 h. *p < 0.05; **p < 0.01: compared with control. ##p < 0.01: compared with cholesterol at the same concentration. $p < 0.05 and $$p < 0.01: compared with CQ. e Immunofluorescence staining of LC3B and p62 in HepG2 cells treated with cholesterol (50 µg/ml), CQ (50 µg/ml), BBR (20 µg/ml), or their combinations for 24 h

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