Fig. 6: COX2 expression and autophagic flux in BBR-treated HepG2 cells. | Cell Death & Disease

Fig. 6: COX2 expression and autophagic flux in BBR-treated HepG2 cells.

From: Berberine ameliorates blockade of autophagic flux in the liver by regulating cholesterol metabolism and inhibiting COX2-prostaglandin synthesis

Fig. 6

a Confocal microscopy examination of HepG2 cells expressing a tandem GFP-RFP-LC3 fusion protein that were treated with cholesterol (Chol, 50 µg/ml) with/without celecoxib (Cel; 50 µM), or subjected to lentivirus-mediated overexpression of COX2 protein with/without berberine (BBR, 20 µg/ml) treatment for 24 h. b Protein levels of COX2, p-AKT, LC3II, and p62 in HepG2 cells treated with cholesterol or lentivirus-mediated overexpression of COX2 with BBR for 24 h. **p < 0.01 compared with control. #p < 0.05 and ##p < 0.01 compared with LV-COX2. c, d Expression of COX2 protein in HepG2 cells treated with cholesterol, cholesterol + Cel, or BBR for 24 h. **p < 0.01 compared with control. ##p < 0.01 compared with cholesterol. e Expression of p-AKT, p62, and LC3II proteins in HepG2 cells treated with different concentrations of PGE2 alone or together with BBR for 24 h. *p < 0.05 and **p < 0.01 compared with control. ##p < 0.01 compared with PGE2 25. $$p < 0.01 compared with PGE2 50

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