Fig. 1: ATF3 deficiency exacerbates hepatocellular damage in IR-induced liver injury. | Cell Death & Disease

Fig. 1: ATF3 deficiency exacerbates hepatocellular damage in IR-induced liver injury.

From: Loss of ATF3 exacerbates liver damage through the activation of mTOR/p70S6K/ HIF-1α signaling pathway in liver inflammatory injury

Fig. 1

Mice were subjected to 90 min of partial liver warm ischemia, followed by 6 h of reperfusion. a Western blot analysis of ATF3 protein expression in hepatocytes and macrophages during IR. Representative histological staining (H&E) of ischemic liver tissue (n = 4–6/group). Original magnification x100. Scale bars = 50 μm. b Liver damage, evaluated by Suzuki’s score. ***p < 0.001. c Hepatocellular function, as assessed by serum ALT levels (IU/L). Results are expressed as the mean ± SD (n = 4–6 samples/group), ***p < 0.001. d Liver neutrophil accumulation, as determined by MPO activity (U/g). Mean ± SD (representative of 4–6 mice/group). **p < 0.01. e, f Liver apoptosis analyzed by TUNEL staining. Results were scored semi-quantitatively by averaging the number of apoptotic cells (mean ± SD) per field at ×400 magnification. Representative of 4–6 mice/group, ***p < 0.001. g Western blot analysis of BCL-2 and BCL-xL. β-actin served as an internal control. Data are representative of three experiments. h Caspase-3 activity. Results are expressed as the mean ± SD (n = 4–6 samples/group), ***p < 0.001

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