Fig. 3: Telomere length and telomeric DNA damage in T cells from HCV patients and HS. | Cell Death & Disease

Fig. 3: Telomere length and telomeric DNA damage in T cells from HCV patients and HS.

From: Inhibition of TRF2 accelerates telomere attrition and DNA damage in naïve CD4 T cells during HCV infection

Fig. 3

a Telomere attrition in CD4 T cells from HCV patients. PBMCs were isolated from 24 HCV-infected patients and 24 age-matched HSs (with or without TCR stimulation), followed by Flow-FISH analysis for telomere length in total CD4, naïve and memory CD4 T cells. The gating strategy, overlaid histograms (dashed lane = isotype control; solid lane = HCV patient; filled area = HS) and summary data of mean fluorescence intensity (MFI) are shown. Each symbol represents one subject. The relative MFI of HCV telomere length was normalized by HS. b Telomeric DNA damage in naïve CD4 T cells derived from chronically HCV-infected individuals versus HS was analyzed by confocal microscopy for colocalization of 53BP1 and TRF1. Representative imaging and summary data for the numbers of TIFs per nuclear, as well as the percentage of cells with > 3 TIFs are shown (n = 12)

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