Fig. 1: PARP12 interacts with FHL2. | Cell Death & Disease

Fig. 1: PARP12 interacts with FHL2.

From: PARP12 (ARTD12) suppresses hepatocellular carcinoma metastasis through interacting with FHL2 and regulating its stability

Fig. 1

a FHL2 was identified to be a PARP12-associated protein by affinity purification. Proteins identified in the PARP12 affinity purification complexes are listed with the number of unique peptides found and the coverage according to mass spectrometry analysis. b, c HA-FHL2 and SFB-PARP12 or GFP-PARP12 and SFB-FHL2 were co-transfected into HEK293T cells and applied to immunoprecipitation (IP) followed by Western blot using the indicated antibodies. Whole-cell lysates were blotted and shown as input. d Endogenous PARP12 interacts with FHL2. Lysates from HEK293T cells were subjected to IP and Western blot with the indicated antibodies. An irrelevant IgG was used as the negative control. *: non-specific bands

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