Fig. 6: Encapsulated mirin reduces tumor growth and induces DDR and apoptosis in vivo.

a Nude mice were subcutaneously inoculated with LAN5 cells. Mice were randomized and injected with 50 mg/kg of mirin^e or vehicle (empty nanoparticles) according to the indicated schedule (arrowheads), for a total of 1.25 mg of mirin^e/mouse. Tumor growth was monitored with the caliper every other day. Data represent the fold increase of the mean tumor volume compared to day 0, for each treatment group (n = 5) ±SEM. b Representative images of xenografted mice at day 11, before tumor explant. c Graph represents the mean volume of the excised tumors ±SEM. p was calculated by the Student’s t-test. *p < 0.05. d Histology, TUNEL assay, and immunostaining for γH2AX and p53^S15P performed on tissue sections from tumor xenografts. Scale bar, 50 μm. e WB showing the expression level of the indicated proteins and phosphoepitopes in two representative tumors excised from mirin^e- or vehicle-treated mice