Fig. 1: CDK5 is activated with Tyr15 phosphorylation and p35 cleavage in ischemic injury. | Cell Death & Disease

Fig. 1: CDK5 is activated with Tyr15 phosphorylation and p35 cleavage in ischemic injury.

From: Zinc induces CDK5 activation and neuronal death through CDK5-Tyr15 phosphorylation in ischemic stroke

Fig. 1

a CDK5 is activated in hippocampus of MCAO rats. Brain tissue homogenates were prepared from rat hippocampus of Sham and MCAO rats (artery occlusion for 60 min, followed with 24-h reperfusion). CDK5 activities were detected by incubating the immunoprecipitated CDK5 from the homogenates with its substrate Histone-H1 and ATP. At the end of incubation, the levels of phosphorylated and total Histone-H1 were shown by western blotting. b CDK5 activities were evaluated by calculating the ratio of phosphorylated to total Histone-H1 levels in a (n = 6 animals per group, Sham vs MCAO, t-test, p = 2.673E-05, ***p < 0.001). c Phosphorylation of CDK5 at Tyr15 and cleavage of p35 to p25 are increased in hippocampus of MCAO rats. Levels of total and Tyr15 phosphorylated CDK5, p35 and p25 in brain homogenates were detected by using the indicated antibodies. d Quantitative analysis of the protein levels in c (n = 6 animals per group, t-test, from left to right, p = 0.0034, p = 0.0015, p = 0.0149, *p < 0.05, **p < 0.01)

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