Fig. 3: Assessment of KRAS mutations in plasma samples.

Bar charts show merged data from two runs for each patient sample. The number of WT (green bars) and mutant (blue bars) copies detected are shown on left y-axis. The total number of droplets analyzed (gray dots) are indicated on right y-axis. a KRAS-c.35; b KRAS-c.34; c KRAS-c.38. The “+ve con” indicates positive control from 10 ng of genomic DNA isolated from SW480 (c.35G>T/p.G12V) CRC cell line, A549 (c.34G>A/p.G12S) lung cancer cell line, and HCT116 (c.38G>A/p.G13D) CRC cell line. The “–ve con” indicates negative control from 10 ng of hgDNA. NTC no template control