Fig. 1: TNFα pretreatment enhances FasL-induced apoptosis.
From: TNFα sensitizes hepatocytes to FasL-induced apoptosis by NFκB-mediated Fas upregulation
a Caspase-3/-7 activity in total cell lysates of primary mouse hepatocytes, treated with FasL alone for 0–5 h (-TNFα) or pretreated with TNFα for 14 h, followed by treatment with FasL for 0–5 h. b 7-AAD staining of primary hepatocytes treated as under (a). The percentage (%) of cells in the subG1 cell cycle stage (apoptotic cells) are shown. c Morphology of living and apoptotic primary hepatocytes treated with TNFα and FasL as indicated, observed under an inverted phase-contrast microscope, scale bar: 100 µm. Caspase-3/-7 activity in total cell lysates of (d) Hepa1-6 or (e) AML12 cell lines either untreated, treated with TNFα or FasL alone or pretreated with TNFα for 14 h followed by FasL for 4 h. a.u. arbitrary units. (a, b) Values represent the means of at least three independent experiments ± SD. (d, e) Data were obtained from four (d) or three (e) independent experiments; the horizontal line represents the mean
