Fig. 5: TNFα treatment does neither upregulate Fas nor enhance FasL-induced apoptosis in HepG2 cells.
From: TNFα sensitizes hepatocytes to FasL-induced apoptosis by NFκB-mediated Fas upregulation
a Caspase-3/-7 activity in total lysates of HepG2 cells, either untreated, treated with TNFα or FasL alone or pretreated with TNFα for 14 h before adding FasL for 4 h. b FACS analysis of surface Fas expression in HepG2 cells treated with TNFα for 14 h. a.u. arbitrary units. c Anti-phospho-p65, anti-IκBα and anti-Fas western blot analysis of total extracts of HepG2 treated with TNFα for up to 840 min. d Anti-p65 and anti-Fas western blot analysis of total extracts of shRenilla or shp65 HepG2 cells. Tubulin serves as loading controls. e FACS analysis of Fas surface expression using anti-hFas conjugated to APC on shRenilla or shp65 HepG2 cells. (a, b, e) Data were obtained from three or two independent experiments as indicated. The horizontal line represents the mean
