Fig. 3: The expression of MFG-E8 on the mRNA and protein level and its cellular distribution after TBI. | Cell Death & Disease

Fig. 3: The expression of MFG-E8 on the mRNA and protein level and its cellular distribution after TBI.

From: Recombinant milk fat globule-EGF factor-8 reduces apoptosis via integrin β3/FAK/PI3K/AKT signaling pathway in rats after traumatic brain injury

Fig. 3

Experimental TBI model in SD rats and the tissue around the lesion in cortex was sampled for western blot and quantitative real-time PCR analysis in this study (a). The mRNA and protein expressions of MFG-E8 were measured at 6 h, 24 h, 72 h, and 7 days after TBI using western blotting and quantitative real-time PCR (b). The mRNA and protein expressions of MFG-E8 were significantly increased at 24 and 72 h after TBI as compared with the Sham group (c, d). Representative immunofluorescence co-staining images of MFG-E8/GFAP, MFG-E8/Iba-1, MFG-E8/NeuN in the Sham group and TBI group (24 h). Astrocytes, microglial cells, and neuron cells are labeled with GFAP (green), Iba-1 (green), and NeuN (green), respectively. (MFG-E8 = red, DAPI = blue). Diagram of coronal rat brain section showing the location of lesion cavity (red) and photograph region (red squares). The quantitative data are the mean ± SD (n = 9 each; *P < 0.05; **P < 0.01 vs. Sham group; nsP > 0.05). Bar = 20 µm

Back to article page