Fig. 5: The effect of rhMFG-E8 on neuronal apoptosis was evaluated by western blotting and immunofluorescence co-staining at 24 and 72 h after TBI. | Cell Death & Disease

Fig. 5: The effect of rhMFG-E8 on neuronal apoptosis was evaluated by western blotting and immunofluorescence co-staining at 24 and 72 h after TBI.

From: Recombinant milk fat globule-EGF factor-8 reduces apoptosis via integrin β3/FAK/PI3K/AKT signaling pathway in rats after traumatic brain injury

Fig. 5

Western blotting showed that rhMFG-E8 (3 μg) administration decreased the expression of cleaved caspase-3 and increased the expression of Bcl-2 at 24 and 72 h after TBI (a–f). Representative immunofluorescence staining images of NeuN/DAPI (NeuN = green, TUNEL = red) (g), results showed administration of rhMFG-E8 decreased the amount of TUNEL-positive neurons at 24 h after TBI (h). Diagram of coronal rat brain section showing the location of lesion cavity (red) and photograph region (red squares) (i). The quantitative data are the mean ± SD (n = 15 each; *P < 0.05, **P < 0.01 vs. Sham group; **P < 0.01 vs. TBI + Vehicle group; #P < 0.05, ##P < 0.01 vs. TBI + Vehicle group). Bar = 20 µm

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