Fig. 7: The effect of integrin β3 on rhMFG-E8's anti-apoptosis was evaluated by western blotting at 24 h after TBI. | Cell Death & Disease

Fig. 7: The effect of integrin β3 on rhMFG-E8's anti-apoptosis was evaluated by western blotting at 24 h after TBI.

From: Recombinant milk fat globule-EGF factor-8 reduces apoptosis via integrin β3/FAK/PI3K/AKT signaling pathway in rats after traumatic brain injury

Fig. 7

Western blotting showed that administration of rhMFG-E8 increased the expressions p-FAK and p-AKT, which were the downstream factors of integrin β3 receptor (b, c), while had no effect on the level of integrin β3 receptor (a). Treatment with integrin β3 siRNA, the levels of integrin β3 receptor, p-FAK and p-AKT were decreased, meanwhile reversed the role of rhMFG-E8 on anti-apoptosis (d–f). The quantitative data are the mean ± SD (n = 6 each; *P < 0.05, **P < 0.01 vs. TBI + Vehicle group; #P < 0.05, ##P < 0.01 vs. TBI + rhMFG-E8 group; nsP > 0.05)

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