Fig. 8: The role of integrin β3/FAK/PI3K/AKT signaling pathway on rhMFG-E8 of anti-apoptosis was evaluated by western blotting at 24 h after TBI. | Cell Death & Disease

Fig. 8: The role of integrin β3/FAK/PI3K/AKT signaling pathway on rhMFG-E8 of anti-apoptosis was evaluated by western blotting at 24 h after TBI.

From: Recombinant milk fat globule-EGF factor-8 reduces apoptosis via integrin β3/FAK/PI3K/AKT signaling pathway in rats after traumatic brain injury

Fig. 8

Western blotting showed that treatment with PI3K inhibitor, LY294002 (5 and 20 µM), decreased the expressions of p-AKT (a, b), high dosage exhibited better effect, while had no effect on integrin β3 and p-FAK (a, c, d). The expressions of pro-apoptosis-associated proteins, Cleaved caspase-3, and Bax were increased after administration of LY294002 (e, f, h), while the level of anti-apoptosis protein Bcl-2 was decreased (e, g). The quantitative data are the mean ± SD (n = 6 each; *P < 0.05, **P < 0.01 vs. TBI + Vehicle group; #P < 0.05, ##P < 0.01 vs. TBI + rhMFG-E8 group; nsP > 0.05)

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