Fig. 1: FMDV infection decreases NME1 protein expression. | Cell Death & Disease

Fig. 1: FMDV infection decreases NME1 protein expression.

From: Foot-and-mouth disease virus induces lysosomal degradation of NME1 to impair p53-regulated interferon-inducible antiviral genes expression

Fig. 1

PK-15 cells cultured in 35-mm dishes were mock-infected or infected with 1 MOI FMDV. The cells were collected at 0, 2, 4, 6, 8, 10, 12, and 16 hpi. a The transcripts of NME1 and viral RNA were analyzed by qPCR. b Expression of NME1 and viral proteins were detected by Western blotting. The relative abundance of VP1 protein was used as an indicator of viral replication. Relative fold-change in abundance of NME1 in mock-infected or FMDV-infected cells was determined by densitometric analysis and normalized to β-actin. All the above experiments were repeated at least three times

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