Fig. 3: NME1 enhances p53 transcriptional function. | Cell Death & Disease

Fig. 3: NME1 enhances p53 transcriptional function.

From: Foot-and-mouth disease virus induces lysosomal degradation of NME1 to impair p53-regulated interferon-inducible antiviral genes expression

Fig. 3

a HEK-293T cells cultured in 24-well plates were co-transfected with 0.2 μg Myc-vector or 0.2 μg Myc-NME1 and 0.1 μg Flag-vector or 0.1 μg Flag-p53 plasmids, along with 0.1 μg p53 luciferase reporter plasmid. pRL-TK Renilla luciferase reporter plasmid (0.01 μg) was used in the reporter assay to normalize transfection efficiency. Cells were collected at 24 hpt and luciferase activities were measured using the dual-specific luciferase assay kit. b HEK-293T cells cultured in 24-well plates were co-transfected with 0.2 μg Myc-vector or 0.2 μg Myc-NME1 along with 0.1 μg p53 luciferase reporter and 0.01 μg pRL-TK plasmids. The transfected cells were incubated with DMSO or 5-FU (20 μg/mL) at 6 hpt, and luciferase activities were measured at 24 h after incubation. c HEK-293T cells cultured in six-well plates were co-transfected with 2 μg Myc-vector or 2 μg Myc-NME1, and the transfected cells were treated with DMSO or 5-FU (20 μg/mL) at 6 hpt for another 24 h. The mRNA expression levels of p53 target genes ISG20, IRF9, RIG-I, and ISG15 were analyzed by qPCR. All the experiments were repeated three times

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