Fig. 7: FMDV VP4 induces NME1 reduction through lysosomal pathway. | Cell Death & Disease

Fig. 7: FMDV VP4 induces NME1 reduction through lysosomal pathway.

From: Foot-and-mouth disease virus induces lysosomal degradation of NME1 to impair p53-regulated interferon-inducible antiviral genes expression

Fig. 7

a PK-15 cells were transfected with 2 μg Flag-VP4 expressing plasmid. The cells were collected at 0, 12, 24, 36, or 48 hpt. Expression of cellular eIF4G was detected using anti-eIF4G antibody. Expression of Flag-VP4 was detected using anti-Flag antibody. b PK-15 cells were treated with with DMSO or CHX (50 μg/mL) for 0, 2, 4, 6, 8, 10, 12, and 16 h. Expression of NME1 was detected by Western blotting using anti-NME1 antibody. c-e HEK-293T cells grown in 6-well plates were co-transfected with 2 μg Myc-NME1, 2 μg Flag-vector, or Flag-VP4 plasmids in the presence or absence of (c) MG132 (5 or 20 μM), (d) Z-VAD-FMK (10 or 50 μM), (e) CQ (50 or 100 μM) or NH4CL (2.5 or 25 mM). The cells were collected at 24 hpt. Expression of Myc-NME1 was detected by Western blotting using anti-Myc antibody. Expression of Flag-VP4 was detected using anti-Flag antibody. Relative fold-change in abundance of Myc-NME1 protein was determined by densitometric analysis. All the experiments were repeated at least three times

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