Fig. 2: Effect of JAM-A silencing on the proliferation and migration of primary keratinocytes. | Cell Death & Disease

Fig. 2: Effect of JAM-A silencing on the proliferation and migration of primary keratinocytes.

From: JAM-A knockdown accelerates the proliferation and migration of human keratinocytes, and improves wound healing in rats via FAK/Erk signaling

Fig. 2

a Time points selected for the in vitro study. Generally, siRNA duplexes for scramble (Scr) or JAM-A were transfected into keratinocyte cultures on day 0. After 24-h reaction, the mixture was replaced with fresh medium, cells were then cultured for another 24 h followed by different assays to examine the protein expression, cell proliferation, and migration at appointed times. b Western blot showing an ~70% knockdown of JAM-A expression in keratinocytes 2 days after RNAi treatment. Densitometric analysis of JAM-A immunoblotting data normalized against actin with scramble RNAi arbitrarily set at 1. Each bar represents mean ± SD from four experiments. **P < 0.01. c The proliferation of keratinocytes after JAM-A silencing was evaluated by MTT assay at 0, 24, 48 h. Bars represent the mean ± SD, n = 4. d The migration of keratinocytes after JAM-A silencing was evaluated by cell scratch assay. Notably, keratinocytes were pre-treated with mitomycin C for 1 h before scratch assay. Images at 0, 24, and 48 h post-scratching were captured to display the process of gap closure. Scale bars = 100 µm. e Densitometric analysis of the data from scratch assay normalized against the gap length at 0 h in scramble RNAi group, which was designated as 1. Bars are means ± SD, n = 4. *P < 0.05; **P < 0.01

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