Fig. 4: Effect of PF-562271 pre-administration on the expression of selected signaling molecules after JAM-A silencing in keratinocytes. | Cell Death & Disease

Fig. 4: Effect of PF-562271 pre-administration on the expression of selected signaling molecules after JAM-A silencing in keratinocytes.

From: JAM-A knockdown accelerates the proliferation and migration of human keratinocytes, and improves wound healing in rats via FAK/Erk signaling

Fig. 4

Keratinocytes received 2-h pre-incubation with PF-562271 or DMSO (as vehicle control, Veh) followed by JAM-A RNAi. Immunoblotting was performed to investigate the protein level change of the total FAK (a), Erk1/2 (b), JNK (c), Src (d), Akt (e), and p38 (f), also the corresponding phosphorylated molecules. Densitometric analysis on the levels of phosphorylated proteins was normalized against the corresponding total protein levels which was then designated as 1. Bars are the means ± SD, n = 4. **P < 0.01; n.d. not detectable. ‘‘1’’, Scr RNAi + Veh; ‘‘2’’, Scr RNAi + PF-562271; ‘‘3’’, JAM-A RNAi + Veh; ‘‘4’’, JAM-A RNAi + PF-562271

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