Fig. 5: Effect of PF-562271 or PD98059 pre-administration on keratinocyte proliferation and migration after JAM-A silencing. | Cell Death & Disease

Fig. 5: Effect of PF-562271 or PD98059 pre-administration on keratinocyte proliferation and migration after JAM-A silencing.

From: JAM-A knockdown accelerates the proliferation and migration of human keratinocytes, and improves wound healing in rats via FAK/Erk signaling

Fig. 5

a Keratinocytes received 2-h pre-incubation with PF-562271, PD98059, or DMSO followed by JAM-A RNAi. In addition, mitomycin C was used to block the proliferation prior to the scratch. Images at 0, 24, and 48 h post-scratching were captured to display the process of gap closure. Scale bars = 100 µm. b Densitometric analysis of the data from scratch assay normalized against the gap length at 0 h in scramble RNAi group which was designated as 1. Bars are means ± SD, n = 4. *P < 0.05; **P < 0.01. c The proliferation of keratinocytes after JAM-A silencing and drug administration was evaluated by MTT assay at 0, 24, and 48 h. Bars represent the mean ± SD, n = 4. **P < 0.01

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