Fig. 6: Study to assess the effect of JAM-A knockdown on wound healing in vivo. | Cell Death & Disease

Fig. 6: Study to assess the effect of JAM-A knockdown on wound healing in vivo.

From: JAM-A knockdown accelerates the proliferation and migration of human keratinocytes, and improves wound healing in rats via FAK/Erk signaling

Fig. 6

a Time points selected for the in vivo study. Generally, on day 0 the dorsal skin of SD rats received 1 × 1 cm2 full-thickness excision followed by immediate RNAi transfection at the wound edge, the RNAi transfection was repeated one more time on day 2. b Skin tissues at the wound edge from day-5 rats were collected, followed by western blot analysis. Densitometric analysis of JAM-A immunoblotting data normalized against actin with scramble RNAi arbitrarily set at 1. Each bar represents mean ± SD from four experiments. **P < 0.01. c In wound healing assay, DMSO, PF-562271, or PD98059 was co-administrated with siRNA on day 0 and 2. Images showing the healing process of rat dorsal skin wounds were captured and recorded on day 1, 5, and 8. Scale bars = 1 cm. d Densitometric analysis of the in vivo wound healing data normalized against the wound area on day 1 in (Scr RNAi + Veh) group which was designated as 1. Each bar represents the mean ± SD, n = 4. **P < 0.01

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