Fig. 5
Downregulation of GRP78 by miR-495-3p inhibits autophagy in GC MDR cells. a Representative images and b Quantification of LC3 puncta (green) in GC MDR cells transfected with si-GRP78 or si-nc. Scale bars: 50 μM. c Representative images and d Quantification of LC3 puncta (green) in SGC7901/ADR co-transfected with GRP78 3′-UTR-mutant overexpression vector/nc and miR-495-3p/nc. e The expression level of GRP78, LC3BI/II, and P62 in GC MDR cells transfected with si-GRP78 or si-NC were determined by western blotting. f The protein level of GRP78, LC3BI/II, and P62 in SGC7901/ADR co-transfected with GRP78 3′-UTR-mutant overexpression vector/nc and miR-495-3p/nc. All values expressed as mean ± SD, n = 3 for each group. *P < 0.05, **P < 0.01, ***P < 0.001
