Fig. 4: Autophagy effectively downregulated the expression of CD133, Nestin, and GFAP. | Cell Death & Disease

Fig. 4: Autophagy effectively downregulated the expression of CD133, Nestin, and GFAP.

From: Autophagy suppresses self-renewal ability and tumorigenicity of glioma-initiating cells and promotes Notch1 degradation

Fig. 4

a–d U87 and U251 cells were treated the same as in (Picture 2). Protein expressions of CD133, Nestin, and GFAP were detect by western blot. Data are shown as means ± s.d., n = 3, *P < 0.05, **P < 0.01, Student’s t-test. e Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM), and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against CD133 and nestin. Images were captured by laser confocal microscope ( × 400), scale bar = 100 μm

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