Fig. 6: Autophagy regulated Notch1 degradation. | Cell Death & Disease

Fig. 6: Autophagy regulated Notch1 degradation.

From: Autophagy suppresses self-renewal ability and tumorigenicity of glioma-initiating cells and promotes Notch1 degradation

Fig. 6

a U87 and U251 GICs were treated with AZD8055 (0.3 µM) for 48 h and rapamycin (3 µM) for 24 h. Cells were immunostained for Notch1 (green) and LC3 (red) and stained with DAPI (blue). The white box indicates the location of the enlargement. The white triangle shows LC3B and Notch1 colocalization with some yellow points. Scale bar = 5 µm. b Cells were immunostained for NICD (green) and LC3B (red) and stained with DAPI (blue) after the same treatment as a. Scale bar = 5 µm. c The expression of Notch1, DLL1 on the membrane of U87 and U251 GICs were detected by western blot. Drugs treatment were the same as in a. d Bar chart were used to compare the expression of Notch1 in three groups treatment. Data are shown as means ± S.D. n = 3, *P < 0.05, **P < 0.01, Student’s t-test. e Mechanism diagram described the line of Notch pathway activation and the progress of Autophagy regulating Notch1 degradation

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